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05-829X-555

Sigma-Aldrich

Anti-α-Tubulin Antibody, Alexa Fluor™ 555 conjugate

clone DM1A, from mouse, ALEXA FLUOR™ 555

Synonym(s):

Anti-B-ALPHA-1, Anti-LIS3, Anti-TUBA3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

pig, guinea pig, human, avian, mouse, bovine, rat

manufacturer/tradename

Chemicon®
Upstate®

technique(s)

immunofluorescence: suitable

isotype

IgG1

NCBI accession no.

NM_006009

UniProt accession no.

Q71U36

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

Specificity

Recognizes α-Tubulin

Immunogen

The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Application

Anti-α-Tubulin Antibody, Alexa Fluor 555 conjugate detects level of α-Tubulin & has been published & validated for use in IF.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Routinely evaluated by immunofluorescence

Target description

50-60 kDa

Physical form

Protein G Purified
Protein G purified immunoglobulin conjugated to Alexa Fluor™ 555. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

Storage and Stability

Maintain at 4°C for up to one year from date of receipt.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sungjin Park et al.
STAR protocols, 4(4), 102562-102562 (2023-09-10)
Traditionally, midbody remnants (MBRs) are isolated from cell culture medium using ultracentrifugation, which is expensive and time consuming. Here, we present a protocol for isolating MBRs or large extracellular vesicles (EVs) from mammalian cell culture using either 1.5% polyethylene glycol
New microtubule/tubulin-targeted anticancer drugs and novel chemotherapeutic strategies.
Wilson, L and Jordan, M A
Journal of Chemotherapy (Florence, Italy), 16 Suppl 4, 83-85 (2004)
Crystallographic structure of tubulin: implications for dynamics and drug binding.
Downing, K H and Nogales, E
Cell Structure and Function, 24, 269-275 (1999)
Erich J Kushner et al.
Molecular biology of the cell, 27(12), 1911-1920 (2016-04-22)
Blood vessel formation requires dynamic movements of endothelial cells (ECs) within sprouts. The cytoskeleton regulates migratory polarity, and centrosomes organize the microtubule cytoskeleton. However, it is not well understood how excess centrosomes, commonly found in tumor stromal cells, affect microtubule

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