05-465
Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1
clone C5/1.1, Upstate®, from mouse
Synonym(s):
G-protein coupled receptor kinase 2, adrenergic, beta, receptor kinase 1, beta adrenergic receptor kinase 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
C5/1.1, monoclonal
species reactivity
rat, mouse, bovine, chicken, human, rabbit, pig
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
chicken ... Grk2(426000)
human ... GRK2(156)
mouse ... Grk2(282682) , Grk2(110355)
pig ... Grk2(100513842)
rat ... Grk2(25238)
General description
The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors.
GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors.
Specificity
This antibody recognizes GRK2/3, Mr 80 kDa. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody.
Immunogen
Epitope: a.a. 483-485
Partial GST fusion protein corresponding to the GRK3 (βARK2) carboxyl terminus (a.a. 467-688). This immunogen shares homology with GRK2 (βARK1). The minimal epitope recognized by the antibody is a.a. 483-485. Clone C5/1.1.
Application
Detect GRK 2/3 (βARK 1/2) using this Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 validated for use in IP & WB.
Immunoprecipitation:
A previous lot of this antibody was used to immunoprecipitate GRK2/3 from transfected cells, as shown by an independent laboratory.
A previous lot of this antibody was used to immunoprecipitate GRK2/3 from transfected cells, as shown by an independent laboratory.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Quality
Routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts, bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma or human HeLa cell lysates.
Western Blot Analysis:
0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates.
Western Blot Analysis:
0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates.
Target description
80 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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L Iacovelli et al.
Molecular pharmacology, 65(5), 1103-1110 (2004-04-23)
We examined the role of G-protein coupled receptor kinase-2 (GRK2) in the homologous desensitization of mGlu4 metabotropic glutamate receptors transiently expressed in human embryonic kidney (HEK) 293 cells. Receptor activation with the agonist l-2-amino-4-phosphonobutanoate (l-AP4) stimulated at least two distinct
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