906905
TissueFab® bioink
Sacrificial
Synonym(s):
PEG-PPG-PEG, Poloxamer F127, Sacrificial bioink
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About This Item
UNSPSC Code:
12352201
NACRES:
NA.23
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description
0.2 μm sterile filtered
suitable for 3D bioprinting applications
form
viscous liquid
impurities
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
color
colorless to pale yellow
pH
6.5-7.5
application(s)
3D bioprinting
storage temp.
2-8°C
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General description
3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
Application
Polaxomer is a thermo-responsive hydrogel which has been used as a mold, track patterning and sacrificial material for bioprinting and tissue engineering. It is considered one of the best printable hydrogels due to the nature of micellar-packing gelation, which allows it to be moved and shifted easily. Moreover, the viscosity of TissueFab®- Sacrificial bioink is stable at both room temperature and human body temperature, which allows it to print at both room temperature and physiological temperature. TissueFab®- Sacrificial Bioink becomes liquid at 4°C and dissolves in aqueous environments, which makes it easy to remove for using as sacrificial material to engineer channels and vasculature in tissue engineering application.
Packaging
Product contains 10 ml of solution packaged in glass bottle.
Other Notes
Important tips for optimal bioprinting results
Procedure
1. Prepare bioink: Keep TissueFab® - Sacrificial Bioink on ice to prevent gelation and gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously. Transfer TissueFab® - Sacrificial Bioink into the desired printer cartridge.
2. Bioprint: Warm TissueFab® - Sacrificial Bioink in the printer cartridge to room temperature for 10–15 minutes to induce gelation. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. TissueFab® - Sacrificial Bioink can be printed in tandem with cell laden bioinks using additional printheads.
3. Optional Crosslink: If additional bioinks are used, crosslink the bioprinted structure before removing sacrificial scaffold following bioink instructions.
4. Remove sacrificial scaffold: Cool printed structure to 4 °C for at least 5 minutes. Rinse or perfuse with cold PBS.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
Procedure
1. Prepare bioink: Keep TissueFab® - Sacrificial Bioink on ice to prevent gelation and gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously. Transfer TissueFab® - Sacrificial Bioink into the desired printer cartridge.
2. Bioprint: Warm TissueFab® - Sacrificial Bioink in the printer cartridge to room temperature for 10–15 minutes to induce gelation. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. TissueFab® - Sacrificial Bioink can be printed in tandem with cell laden bioinks using additional printheads.
3. Optional Crosslink: If additional bioinks are used, crosslink the bioprinted structure before removing sacrificial scaffold following bioink instructions.
4. Remove sacrificial scaffold: Cool printed structure to 4 °C for at least 5 minutes. Rinse or perfuse with cold PBS.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
Legal Information
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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Michael Müller et al.
Journal of visualized experiments : JoVE, (77), e50632-e50632 (2013-07-31)
Bioprinting is an emerging technology that has its origins in the rapid prototyping industry. The different printing processes can be divided into contact bioprinting(1-4) (extrusion, dip pen and soft lithography), contactless bioprinting(5-7) (laser forward transfer, ink-jet deposition) and laser based
Maja Radivojša Matanović et al.
International journal of pharmaceutics, 472(1-2), 262-275 (2014-06-21)
Thermally induced gelling systems have gained enormous attention over the last decade. They consist of hydrophilic homopolymers or block copolymers in water that present a sol at room temperature and form a gel after administration into the body. This article
Bioinks for biofabrication: current state and future perspectives.
Prendergast M E, et al.
Journal of 3D printing in medicine, 1, 49-62 (2016)
Sean V Murphy et al.
Nature biotechnology, 32(8), 773-785 (2014-08-06)
Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as engineering, manufacturing, art, education and medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional
David B Kolesky et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(12), 3179-3184 (2016-03-10)
The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin
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