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T8778

Sigma-Aldrich

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym(s):

α,α-Trehalose glucohydrolase

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About This Item

CAS Number:
9025-52-9
Enzyme Commission number:
3.2.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00132462
UNSPSC Code:
12352204
NACRES:
NA.54
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form

buffered aqueous glycerol solution

Quality Level

200

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

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General description

Trehalase is a glycosidase[1] that occurs in many forms and is found in all tissues.[2] It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.[1][3]
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus. [4]

Application

Trehalase has been used:
  • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves[5]
  • in a study to investigate growth arrest by trehalose-6-phosphate[6]
  • to quantify the intracellular trehalose in Lactobacillus casei cells[7]

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.[2]

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000280313
0000352066
0000352227
0000280313
0000352066

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Shari Dhaene et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 1964-1989 (2020-11-10)
Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose
Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Berrak Gülçin Balaban et al.
Microorganisms, 8(1) (2019-12-28)
Iron plays an essential role in all organisms and is involved in the structure of many biomolecules. It also regulates the Fenton reaction where highly reactive hydroxyl radicals occur. Iron is also important for microbial biodiversity, health and nutrition. Excessive
Choowong Auesukaree et al.
Journal of bioscience and bioengineering, 114(2), 144-149 (2012-05-15)
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723
Wim Schepers et al.
The Journal of biological chemistry, 287(53), 44130-44142 (2012-11-17)
The readdition of an essential nutrient to starved, fermenting cells of the yeast Saccharomyces cerevisiae triggers rapid activation of the protein kinase A (PKA) pathway. Trehalase is activated 5-10-fold within minutes and has been used as a convenient reporter for
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How much volume of trehalase enzyme is provided in a vial o1 1 Unit enzyme. Could you share the technical bulletin of trehalase.

    1 answer

    1. The volume of this product is lot specific and may be calculated using data provided in the Certificate of Analysis. For example, a reported protein concentration of 0.8 mg protein/mL with an activity of 2.6 units/mg protein would have a volume of 384 uL for the 1 unit pack size and 1.92 mL for the 5 unit pack size. A datasheet is not provided for this item. Please see the links below to review other helpful documentation.

      Sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/t8778#product-documentation

      Enzymatic assay method:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/120/796/t8778enz.pdf

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  2. What is the molecular weight of Product T8778, Trehalase from porcine kidney?

    1 answer

    1. The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979).

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. How is Product T8778, Trehalase from porcine kidney prepared?

    1 answer

    1. The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).

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