Solutions of lyticase can be prepared in DNA-free water. One publication reports the preparation of stock solutions of lyticase at 5 mg/mL in 1 M sorbitol with 0.1 M EDTA, pH 8.0, with storage of these stock solutions at –20 C in frozen aliquots. However, this method has not been tested by us. The lyticase may also be dissolved in 50 mM potassium phosphate buffer, pH 7.5, with 50% glycerol and stored at -20 deg. Solutions of Lyticase are not considered to be stable, and it is recommended that solutions be prepared fresh and not stored as stock solutions. We do not have information on the long-term stability if stored at -20 C. We would advise using it within a period of 1 month if stored at -20 C in aliquots.
SAE0098
Lyticase from Arthrobacter luteus
free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
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biological source
bacterial (Arthrobacter luteus)
form
lyophilized powder
specific activity
≥2000 units/mg protein
feature
DNA free
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
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General description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Unit Definition
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What recommendations are available for the reconstitution of Lyticase? Should it be prepared in water or a buffer? Can you provide general information as well as specific information for product SAE0098?
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