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SAB4200819

Sigma-Aldrich

Anti-Connexin 43 antibody, Mouse monoclonal

clone CXN-6, purified from hybridoma cell culture

Synonym(s):

Cx43, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CXN-6, monoclonal

mol wt

~43 kDa

species reactivity

porcine, feline, chicken, mouse, human, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL
immunofluorescence: 10-20 μg/mL

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Porcine ... GJA1(100518636)
chicken ... GJA1(395278)
feline ... GJA1(101100211)
human ... GJA1(2697)
mouse ... GJA1(14609)
rat ... GJA1(24392)

General description

Monoclonal Anti-Connexin 43 (mouse IgM isotype) is derived from the hybridoma CXN-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide from the C-terminal region of Connexin 43 protein, conjugated to KLH. Connexin 43 protein (Cx43), also known as Gap junction α-1 protein or Gap junction 43 kDa heart protein. It has four transmembrane domains (TMs). The N and C-terminal cytoplasmic tails orient in plasma membrane. It also has two extracellular loop (EL) domains and a cytoplasmic loop (CL). Six connexin subunits form a connexon or hemichannel in the plasma membrane and a head-to-head docking between two hemichannels result in the formation of a gap junction channel. Cx43 is expressed in heart and brain. It is expressed in cell types including the melanocytes, keratinocytes, endothelial and basal cells. Cx43 has been mapped to human chromosome 6q22.31.

Specificity

Monoclonal Anti-Connexin 43 specifically recognizes Connexin 43 from human1,2, mouse2, rat3, porcine4, chicken5 and feline6 origin. 

Immunogen

Synthetic peptide corresponding to human connexin-43 C-terminal region, conjugated to KLH

Application

The antibody may be used in various immunochemical techniques including Immunoblot (~43 kDa), Immunohistochemistry and Immunofluorescence. 

Biochem/physiol Actions

Connexin 43 (Cx43) functions as a neuroprotector and is most prominently expressed in astrocytes and microglial cells in the brain. It plays a key role in intercellular communication. Cx43 mediates synchronized contraction of the heart. It modulates of the cell migration and cell adhesion processes. Altered connexin 43 expression is implicated in hypertrophic cardiomyopathy. It is less expressed in human tumors. It is a potential target for treating skin disorders. Its downregulation favours wound healing.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of connexin 43 in different forms of intercellular communication-gap junctions, extracellular vesicles and tunnelling nanotubes
Ribeiro-Rodrigues TM, et al.
Journal of Cell Science, 130(21), 3619-3630 (2017)
Increased connexin 43 expression as a potential mediator of the neuroprotective activity of the corticotropin-releasing hormone
Hanstein R, et al.
Molecular Endocrinology, 23(9), 1479-1493 (2009)
Gap junction-mediated transfer of miR-145-5p from microvascular endothelial cells to colon cancer cells inhibits angiogenesis
Thuringer D, et al.
Testing, 7(19), 28160-28160 (2016)
Role of connexin 43 in cardiovascular diseases
Michela P, et al.
European Journal of Pharmacology, 768, 71-76 (2015)
Genetics of vascular malformations
Seminars in Pediatric Surgery, 23(4), 221-226 (2014)

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