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SAB4200068

Sigma-Aldrich

Anti-PSPC1 (N-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-PSP1, Anti-Paraspeckle protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~59 kDa

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1.2-2.5 μg/mL using paraformaldehyde fixed HEK-293T cells
western blot (chemiluminescent): 0.5-1 μg/mL using lysates of HEK-293T cells

UniProt accession no.

Q8WXF1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PSPC1(55269)

Related Categories

General description

Paraspeckle protein 1 (PSP1/ PSPC1) has two isoforms, α and β. PSPC1 belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family.

Application

Anti-PSPC1 (N-terminal) antibody produced in rabbit may be used in several immunochemical techniques including immunoblotting (~59 kDa) and immunofluorescence. It has also been used in RNA immunoprecipitation (RIP) experiment.

Biochem/physiol Actions

Paraspeckle protein 1(PSP1/ PSPC1) is implicated in the regulation of nuclear receptors such as the progesterone receptor and the thyroid receptor, and in a wide range of processes such as transcription, splicing, and RNA metabolism. It acts as a marker for paraspeckles, which are ribonucleoprotein bodies found in the interchromatin space of mammalian cell nuclei that are often located adjacent to splicing speckles.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The DBHS proteins SFPQ, NONO and PSPC1: a multipurpose molecular scaffold
Knott GJ, et al.
Nucleic Acids Research, 44(9), 3989-4004 (2016)
Circadian RNA expression elicited by 3?-UTR IRAlu-paraspeckle associated elements
Torres M, et al.
eLife, 5(9), e14837-e14837 (2016)
Manon Torres et al.
eLife, 5 (2016-07-22)
Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA
P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner
Fox AH, et al.
Molecular Biology of the Cell, 16(11), 5304-5315 (2005)
Pei Wen Lee et al.
The Journal of biological chemistry, 298(11), 102563-102563 (2022-10-10)
RNA-binding proteins of the DBHS (Drosophila Behavior Human Splicing) family, NONO, SFPQ, and PSPC1 have numerous roles in genome stability and transcriptional and posttranscriptional regulation. Critical to DBHS activity is their recruitment to distinct subnuclear locations, for example, paraspeckle condensates
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