P3118

Monoclonal Anti-p115/TAP antibody produced in mouse

clone 5D6, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Transcytosis Associated Protein
• MDL number: MFCD03792426
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5D6, monoclonal
• form: buffered aqueous solution
• species reactivity: rat
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver
• isotype: IgG1
• UniProt accession no.: P41542
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Uso1(56042)

Description

General description

Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain.

Specificity

Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa).

Immunogen

purified rat p115/TAP.

Application

Monoclonal Anti-p115/TAP antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra−Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.