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P1588

Sigma-Aldrich

Placenta Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):

PGF, PlGF

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About This Item

MDL number:
MFCD00677685
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Placenta Growth Factor human, PlGF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

powder

quality

endotoxin tested

mol wt

29 kDa

packaging

pkg of 10 and 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

Biochem/physiol Actions

Placenta growth factor (PlGF) is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Preparation Note

Recombinant human PlGF is lyophilized from a 0.2 micron filtered solution of 30% CH3CN and 0.1% TFA (pH 7.4) containing 50 microg of bovine serum albumin per 1 microg of cytokine.

Reconstitution

Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.

Analysis Note

The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Domenico Ribatti
Angiogenesis, 11(3), 215-221 (2008-06-24)
The placental growth factor (PlGF) is an angiogenic protein belonging to the vascular endothelial growth factor (VEGF) family, which was discovered in 1991 by an Italian scientist, Maria Graziella Persico. Dr Persico cloned and purified PlGF and determined its structure
Ai-Yi Zhou et al.
World journal of pediatrics : WJP, 10(3), 262-270 (2014-08-16)
Clinical trials have revealed that the antivascular endothelial growth factor (VEGF) therapies are effective in retinopathy of prematurity (ROP). But the low level of VEGF was necessary as a survival signal in healthy conditions, and endogenous placental growth factor (PIGF)
Suk Ho Byeon et al.
Investigative ophthalmology & visual science, 51(2), 1190-1197 (2009-10-17)
Vascular endothelial cell growth factor (VEGF) is strongly induced by oxidative stress in retinal pigment epithelial (RPE) cells, and VEGF-A is a survival factor for various cell types. This study was conducted to determine whether the autocrine VEGF signaling pathway
G Conn et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(4), 1323-1327 (1990-02-01)
A growth factor that is mitogenic for vascular endothelial cells, with an ED50 of approximately 1 ng/ml, has been purified 170,000-fold to apparent homogeneity from tissue culture medium conditioned by a rat glioma-derived cell line. The pure protein is a
Xu Zhou et al.
Scientific reports, 5, 10071-10071 (2015-05-12)
Cancer neovascularization plays an essential role in the metastasis of larynx carcinoma (LC). However, the underlying molecular mechanisms are not completely understood. Recently, we reported that placental growth factor (PLGF) regulates expression of matrix metalloproteinase 3 (MMP3) through ERK/MAPK signaling
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