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MAK127

Sigma-Aldrich

Copper Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Copper Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

orthopedic diseases; aging/geriatric diseases; hematological disorder; gastrointestinal diseases; neurological disorders; dermatological diseases

storage temp.

2-8°C

Related Categories

General description

Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson′s disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.

Application

Copper Assay Kit has been used to determine the copper content colorimetrically in samples.[1]

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of of copper in biological, environmental, food, and beverage samples. Suitable for studying the effects of compounds on copper metabolism.

Principle

The method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured colorimetrically (359 nm), is directly proportional to copper concentration in the sample. The range of linear detection is 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM).

Signal Word

Danger

Hazard Statements

H290,H314,H335,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
127CE08A15
127CE08A15
127CE03A14
127CD01A05
127CD04A20

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Estimating global enzyme abundance levels from cofactor requirements: a model-based analysis of the iron metabolism in yeast.
Dikicioglu D and Oliver SG
bioRxiv, 229104-229104 (2017)
Ryan Philip Henry Shaw et al.
Endocrinology, 163(6) (2022-04-23)
Small heterodimer partner (Shp) regulates several metabolic processes, including bile acid levels, but lacks the conserved DNA binding domain. Phylogenetic analysis revealed conserved genetic evolution of SHP, FXR, CYP7A1, and CYP8B1. Shp, although primarily studied as a downstream target of
Gabriela Fernandes et al.
The journal of adhesive dentistry, 22(3), 265-274 (2020-05-22)
To investigate whether dental adhesives modified with polyacrylic acid copper iodide particles could inhibit esterase activity in vitro and the copper release rate from resin matrices, as well as the correlation between the two variables. Different concentrations of copper iodide
Jereme G Spiers et al.
Antioxidants (Basel, Switzerland), 11(1) (2022-01-22)
Essential metals such as copper, iron, and zinc are cofactors in various biological processes including oxygen utilisation, cell growth, and biomolecular synthesis. The homeostasis of these essential metals is carefully controlled through a system of protein transporters involved in the
Changfeng Li et al.
Developmental cell, 46(4), 441-455 (2018-08-14)
Pancreatic cancer is an aggressive malignancy with changes in the tumor microenvironment. Here, we demonstrate that PINK1 and PARK2 suppressed pancreatic tumorigenesis through control of mitochondrial iron-dependent immunometabolism. Using mouse models of spontaneous pancreatic cancer, we show that depletion of Pink1 and Park2

Questions

1–5 of 5 Questions  

  1. 该产品是否可以检测脑组织中的铜离子，脑组织是否需要特殊处理？

    1 answer

    1. This assay kit has not been specifically tested on brain tissue. Researchers have published using this kit with brain tissue. Their sample preparation involved homogenizing brain tissue in distilled water prior to centrifugation and assaying the supernatant for copper content. Please see the citation below for more details.

      Ma X, Sun Y, Li C, Wang M, Zang Q, Zhang X, Wang F, Niu Y, Hua J. Novel Insights Into DLAT's Role in Alzheimer's Disease-Related Copper Toxicity Through Microglial Exosome Dynamics. CNS Neurosci Ther. 2024 Oct;30(10):e70064. doi: 10.1111/cns.70064. PMID: 39428563; PMCID: PMC11491298.

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  4. How to homogenize the mouse liver, for example, in what buffer, to measure copper content using the MAK127 Copper Assay Kit?

    1 answer

    1. This is the closest protocol for liver homogenate for use of the kit with cell lysates (presumably cultured cells):
      - Lyse cells in 0.5 N NaOH for 30 minutes on a shaking plate followed by a 30-minute incubation at 100 degrees C.
      - Clarify the lysate by centrifugation (2 min at 14,000 rpm) and use the clear supernatant for the assay.
      - Avoid the use of divalent chelators like EDTA during the preparation.
      It indicates that Reagent A contains Trichloroacetic acid (concentration not provided), so it should neutralize the NaOH in the lysate. It is recommended to review the kit's Technical Bulletin regarding the possible need to centrifuge samples that contain proteins.

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  5. Does this product solely detect free copper in the solution, or does it also measure bound copper, such as Ceruloplasmin-bound copper?

    1 answer

    1. This kit is not specifically validated for ceruloplasmin-bound copper, But, it is believed that the assay can measure ceruloplasmin-bound copper because it uses a very strong copper chelate. Any other means to dissociate the Cu (such as heating to denature protein) would be helpful, although they may not be necessary.

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