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G4296

Sigma-Aldrich

GSK3β, active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Pricing and availability is not currently available.

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

143-193 nmol/min·mg

mol wt

~48 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

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manufacturer/tradename

Goodfellow 971-808-70

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manufacturer/tradename

Goodfellow 151-298-17

assay

≥99.9%

assay

99.9%

assay

99.90%

assay

99.90%

mp

2996 °C (lit.)

mp

2996 °C (lit.)

mp

2996 °C (lit.)

mp

2996 °C (lit.)

resistivity

13.5 μΩ-cm, 20°C

resistivity

13.5 μΩ-cm, 20°C

resistivity

13.5 μΩ-cm, 20°C

resistivity

13.5 μΩ-cm, 20°C

bp

5425 °C (lit.)

bp

5425 °C (lit.)

bp

5425 °C (lit.)

bp

5425 °C (lit.)

General description

Research area: Cellsignalling. GSK3B (glycogen synthase kinase 3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria.[1] It is a protein-serine kinase.[2] This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.[3]

Application

GSK3β, active, His tagged human has been used as an incubating medium during in vitro hyperphosphorylation of tau protein.[4][5][6]

Biochem/physiol Actions

GSK3β is a serine-threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase.GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes,[7] including the control of glycogen and protein synthesis by insulin and modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3β into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies.
Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286.[8] It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues.[2] This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins which are linked with Alzheimer′s disease (AD).[3]

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Glycogen synthase kinase 3 in Wnt signaling pathway and cancer
Nydia Tejeda-Mu?oz
IUBMB Life (2015)
Lannah S Abasi et al.
Communications biology, 7(1), 251-251 (2024-03-02)
Tau is a microtubule-associated protein often found in neurofibrillary tangles (NFTs) in the brains of patients with Alzheimer's disease. Beyond this context, mounting evidence suggests that tau localizes into the nucleus, where it may play a role in DNA protection
C Sutherland et al.
The Biochemical journal, 296 ( Pt 1), 15-19 (1993-11-15)
The beta-isoform of glycogen synthase kinase-3 (GSK3 beta) isolated from rabbit skeletal muscle was inactivated 90-95% following incubation with MgATP and either MAP kinase-activated protein kinase-1 (MAPKAP kinase-1, also termed RSK-2) or p70 S6 kinase (p70S6K), and re-activated with protein
Bettina M Foidl et al.
Frontiers in aging neuroscience, 10, 113-113 (2018-05-05)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder of the brain, characterized by extracellular aggregation of beta-amyloid (Aβ) and hyperphosphorylation of tau causing intraneuronal neurofibrillary tangles (NFTs). There is urgent need to study the interactions between Aβ and tau, especially
T Hennet
Cellular and molecular life sciences : CMLS, 59(7), 1081-1095 (2002-09-12)
Galactose is transferred via several linkages to acceptor structures by galactosyltransferase enzymes. In prokaryotes, galactose is mainly found on lipopolysaccharides and capsular polysaccharides. In eukaryotes, galactosyltransferases, which are localized in the Golgi apparatus, are involved in the formation of several

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