Pricing and availability is not currently available.
Recommended Products
Product Name
DI TNC1, 98102214
biological source
rat brain
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal (astrocyte)
products
Alpha-2 macroglobulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Astrocyte transfected
Cell Line Description
Established by transfecting cultures of primary astrocytes from diencephalon tissue of a 1 day old sprague-dawley rat with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was affected using the human GFAP promoter (pGFA-SV-Tt) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes, including immunoreactivity to glial fibrillary acid protein (GFAP) and a β-alanine inhibitable high-affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactocerebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 90% of cells.
Application
Studies of the development and biochemistry of the central neurosystem
Culture Medium
DMEM + 4mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) at 1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing effectiveness of trypsin.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
