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Key Documents

KYSE-70

94072012, human esophagus, Epithelial

Synonym(s):

KY70 Cells, KYSE70 Cells

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About This Item

UNSPSC Code:
41106514

product name

KYSE-70, 94072012

biological source

human esophagus

growth mode

Adherent

karyotype

Hypotriploid with 8% polyploidy

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human oesophageal squamous cell carcinoma

Cell Line Description

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time ~35 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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