Skip to Content
Merck
All Photos(4)

Documents

B4287

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

Quality Level

200

grade

for molecular biology

product line

BioReagent

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

Protease free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Select the right albumin for your application with the Albumin Selection Guide.
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Protease free
  • Suitable for blocking non-specific protein binding sites in hybridizations
  • Heat shock fractionated

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Robert W Crawford et al.
Infection and immunity, 76(11), 5341-5349 (2008-09-17)
Salmonella enterica serovar Typhi can establish a chronic, asymptomatic infection of the human gallbladder, suggesting that this bacterium utilizes novel mechanisms to mediate enhanced colonization and persistence in a bile-rich environment. Gallstones are one of the most important risk factors
Eva P Browne et al.
Aquatic toxicology (Amsterdam, Netherlands), 92(4), 281-287 (2009-04-07)
Polybrominated diphenyl ethers (PBDEs) are brominated flame retardants that persist in the environment and are present in geographically widespread fish species. PBDE concentrations can be particularly high in resident Chinook salmon (Onchorhynchus tshawytscha) in the Puget Sound, Washington. Although PBDE
Ruth Jinfen Chai et al.
PloS one, 6(12), e28090-e28090 (2011-12-14)
Reasons for the progressive age-related loss of skeletal muscle mass and function, namely sarcopenia, are complex. Few studies describe sarcopenia in mice, although this species is the mammalian model of choice for genetic intervention and development of pharmaceutical interventions for
View All Related Papers

Protocols

30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service