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A3415

Sigma-Aldrich

Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Sheep Antibody, Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey, Anti Sheep Hrp

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About This Item

MDL number:
MFCD00162847
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

donkey

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

sheep

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marcus G Davey et al.
PloS one, 12(1), e0171132-e0171132 (2017-02-01)
A major limitation to adeno-associated virus (AAV) gene therapy is the generation of host immune responses to viral vector antigens and the transgene product. The ability to induce immune tolerance to foreign protein has the potential to overcome this host
Increased contractility of diabetic rabbit corpora smooth muscle in response to endothelin is mediated via Rho-kinase β
Chang, S., et al.
The Journal of Urology, 15, 53-62 (2003)
María J Torres et al.
Frontiers in microbiology, 8, 1621-1621 (2017-09-16)
The powerful greenhouse gas, nitrous oxide (N
ACE inhibition increases expression of the ETB receptor in kidneys of mice with unilateral obstruction.
Moridaira, K., et al.
American Journal of Physiology: Renal Physiology, 284(1), F209-F217 (2003)
S Chang et al.
International journal of impotence research, 15(1), 53-62 (2003-02-28)
Corpus cavernosum smooth muscle (CCSM) from rabbits made diabetic for 6 months as a result of alloxan injection exhibited increased sensitivity (3vs 9 nM EC(50)) and generated 20-50% greater force to endothelin-1 (ET-1) compared to CCSM from normal rabbits. In
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