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biological source
mouse spleen
form
liquid
packaging
tube of 5 μg 99072804-DNA-5UG
pkg of vial of cells 99072804-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
Cell Line Origin
Mouse friend leukemia lymphoblast
Cell Line Description
T-3-Cl-2-0.fl is a clone of T-3-Cl-2, which originated from a splenic lesion of Friend virus-induced leukemia in a DD mouse. The cell line can be induced to differentiate into erythroblastoid cells with 1.5% DMSO. T-3-Cl-2-0.fl has been eradicated from mycoplasma using MC-210 by the originator.
Application
Study of erythroid differentiation
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO2; 37ºC
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Certificates of Analysis (COA)
Lot/Batch Number
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