A8654
Bovine Serum Albumin
dimer mol wt ~132 kDa
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
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biological source
bovine
Quality Level
Assay
0.9-1.4 mg per vial protein basis (biuret)
form
lyophilized powder
mol wt
dimer ~132 kDa
monomer ~66 kDa
~66 kDa
purified by
cold ethanol fractionation
origin
USA origin
concentration
0.9—1.4 mg per vial protein (biuret)
technique(s)
protein purification: suitable
pH
5.2
storage temp.
−20°C
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Application
Marker for non-denaturing PAGE
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Reconstitution
0.9-1.4 mg/mL after reconstitution with 1 mL of water
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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