860867

Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

• Synonym(s): 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C
• Empirical Formula (Hill Notation): C₁₇H₂₀ClN₃O · 0.5ZnCl₂
• CAS Number: 51716-96-2
• Molecular Weight: 385.96
• Beilstein: 5690713
• EC Number: 279-675-0
• MDL number: MFCD00148901
• UNSPSC Code: 12171500
• PubChem Substance ID: 24888521
• NACRES: NA.47

Properties

• Quality Level: 200
• form: powder or crystals
• composition: Dye content, ≥50%
• technique(s): microbe id | staining: suitable
• mp: 233-236 °C (lit.)
• λmax: 622 nm
• ε (extinction coefficient): ≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L; ≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.CC\[N+](CC)=C1\C=CC2=Nc3cc(C)c(N)cc3OC2=C1.CC\[N+](CC)=C4\C=CC5=Nc6cc(C)c(N)cc6OC5=C4
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: SIHASHFMDFXGSX-UHFFFAOYSA-J

Description

Application

Brilliant cresyl blue ALD has been used for the staining of reticulocytes.[1]

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.[2]

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves