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MABF153E

Sigma-Aldrich

Anti-CD8a (Mouse), clone 53-6.7, biotin conjugate Antibody

clone 53-6.7, from rat, purified by affinity chromatography

Synonym(s):

T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, CD8a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

53-6.7, monoclonal

purified by

affinity chromatography

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Cd8A(12525)

General description

The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Application

Anti-CD8a (Mouse), clone 53-6.7, biotin conjugate Antibody detects level of CD8a and has been published and validated for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD8a in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg


Certificates of Analysis (COA)

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