MAB3420A4
Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor™ 488 Conjugate
clone PC1C6, from mouse, ALEXA FLUOR™ 488
Synonym(s):
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PC1C6, monoclonal
species reactivity
mouse, rat
species reactivity (predicted by homology)
bovine (immunogen homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Mapt(17762) , Mapt(281296)
rat ... Mapt(29477)
General description
Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)
Specificity
This antibody binds to all known electrophoretic species of tau in human, rat, and bovine brain (one-dimensional SDS-PAGE).
Immunogen
Purified denatured bovine microtubule associated proteins.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor 488 Conjugate is validated for use in IC, IH for the detection of Tau-1.
Quality
Evaluated by Immunohistochemistry in adult mouse brain tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Tau-1 in adult mouse brain tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Tau-1 in adult mouse brain tissue.
Target description
79 kDa calculated. Uniprot describes isoforms ranging from 33 to 81 kDa
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Adult mouse brain tissue
Adult mouse brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Neuroprotection and axon regeneration by novel low-molecular-weight compounds through the modification of DOCK3 conformation.
Namekata, et al.
Cell death discovery, 9, 166-166 (2023)
Regulation of axon growth by the JIP1-AKT axis.
Dajas-Bailador, F; Bantounas, I; Jones, EV; Whitmarsh, AJ
Journal of Cell Science null
Dongdong Zhang et al.
Nature neuroscience, 27(2), 232-248 (2024-01-04)
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