MAB2059-C
Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free
clone ASC-8, from mouse
Synonym(s):
Integrin beta-4, Integrin B4, Integrin Beta4, GP150, CD104
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ASC-8, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB4(3691)
Related Categories
General description
Integrin proteins couple the ECM outside of a cell to the cytoskeleton inside of the cell. Which ECM protein binds and what signals are conveyed depend upon the particular makeup of the integrin pair being expressed. Functional integrin receptors are made up of two protein chains linked by disulfide bonds, an alpha chain and a beta chain. Integrin beta 4, also known as CD104, or GP160 is one of eight different beta chains, however beta 4 integrin is part of only one principal integrin receptor, integrin alpha6beta4. This receptor is expressed in endothelium, epithelium, and perineurium tissues of several organs as well as fibroblasts and Schwann cells but not in blood or lymphatic cells. Integrin alpha6beta4 is the laminin receptor and is critical for cellular polarity and motility.
Immunogen
SCC9 whole cells expressing Integrin B4.
Application
Immunohistochemistry Analysis: A representative lot detected Integrin B4 in acetone-fixed frozen human epithelial, tongue, foreskin, and ovary tissues. (Note: Not for use on formalin-fixed tissues.)
Inhibition Assay Analysis: A representative lot inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptides, but not to type IV collagen or fibronectin. It does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Flow Cytometry Analysis: A representative lot detected Integrin B4 in SCC9, SKOV-3, HUVEC cells. It also detected Integri B4 in RKO and K562 cells transfected for CD104 expression. It does not stain Integrin B4 in monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin B4 from surface-biotinylated SCC9 cells. Immunoprecitated Integrin B4 was then observed at 66 kDa, 76 kDa, 120 kDa, 135 kDa and 200kDa (reduced), or 66 kDa, 110 kDa, 125 kDa, 130 kDa and 190kDa (non-reduced) in PAGE.
Inhibition Assay Analysis: A representative lot inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptides, but not to type IV collagen or fibronectin. It does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Flow Cytometry Analysis: A representative lot detected Integrin B4 in SCC9, SKOV-3, HUVEC cells. It also detected Integri B4 in RKO and K562 cells transfected for CD104 expression. It does not stain Integrin B4 in monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin B4 from surface-biotinylated SCC9 cells. Immunoprecitated Integrin B4 was then observed at 66 kDa, 76 kDa, 120 kDa, 135 kDa and 200kDa (reduced), or 66 kDa, 110 kDa, 125 kDa, 130 kDa and 190kDa (non-reduced) in PAGE.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free is validated for use in flow cytometry, inhibition, IHC & IP for the detection of Integrin B4.
Quality
Evaluated by Flow Cytometry in A431 cells.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Integrin B4 in 1X10E6 A431 cells.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Integrin B4 in 1X10E6 A431 cells.
Target description
202 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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