MAB16985
Anti-MCAM Antibody, clone P1H12
clone P1H12, Chemicon®, from mouse
Synonym(s):
CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P1H12, monoclonal
species reactivity
mouse, canine, human
should not react with
rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MCAM(4162)
Related Categories
General description
MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
Specificity
Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
Immunogen
Immunization of mice with HUVEC.
Application
Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
Target description
110 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.
Analysis Note
Control
HUVEC cells.
HUVEC cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ariya D Lapan et al.
Methods in molecular biology (Clifton, N.J.), 798, 3-19 (2011-12-02)
Dissociated human fetal skeletal muscle contains myogenic cells, as well as non-myogenic cells such as adipocytes, fibroblasts, and lymphocytes. It is therefore important to determine an efficient and reliable isolation method to obtain a purer population of myoblasts. Toward this
Jennifer Walter-Yohrling et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 10(6), 2179-2189 (2004-03-26)
Identification of appropriate models for in vivo and in vitro preclinical testing of inhibitors of tumor angiogenesis and progression is vital to the successful development of anticancer therapeutics. Although the focus is on human molecular targets, most preclinical in vivo
E Favaro et al.
Diabetologia, 48(12), 2552-2562 (2005-11-18)
Studies on the biology of the microvascular endothelial cells (MECs) that surround and penetrate the pancreatic islets are hampered by difficulties in isolating and culturing large numbers of pure cells. We aimed to morphologically and functionally characterise primary MECs purified
Sujata Kale et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(2), 270-271 (2004-12-08)
The vasculature consists of endothelial cells (ECs) lined by pericyte/vascular smooth muscle cells (vSMCs). Pericyte/vSMCs provide support to the mature vasculature but are also essential for normal blood vessel development. To determine how pericyte-EC communication influences vascular development, we used
Marcie R Finney et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 12(5), 585-593 (2006-04-26)
Endothelial precursor cells (EPCs) cultured from adult bone marrow (BM) have been shown to mediate neovasculogenesis in murine models of vascular injury. We sought to directly compare umbilical cord blood (UCB)- and BM-derived EPC surface phenotypes and in vivo functional
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