USP HPLC Analysis of Ampicillin Sodium on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm × 4.6 mm I.D., 5 μm particles (50536-U)

mobile phase

[A:B:C:D] [A] acetonitrile, [B]water, [C] 1M Potassium phosphate monobasic in water, [D] 1N acetic acid in water (80:909:10:1)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

10 μL

sample

1 mg/mL in mobile phase