์ฝ˜ํ…์ธ ๋กœ ๊ฑด๋„ˆ๋›ฐ๊ธฐ
Merck

LC/MS Analysis of Vaccinium Anthocyanidins on Ascentisยฎ Express C18 at Elevated Temperature and Flow

LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow application for HPLC

Materials

๋ถ„์„ ์ปฌ๋Ÿผ

์ œํ’ˆ ๋ฒˆํ˜ธ
์„ค๋ช…
๊ฐ€๊ฒฉ

Ascentisยฎ Express C18, 2.7 ฮผm HPLC Column

2.7 μm particle size, L ร— I.D. 10 cm ร— 2.1 mm

ํ‘œ์ค€

์ œํ’ˆ ๋ฒˆํ˜ธ
์„ค๋ช…
๊ฐ€๊ฒฉ

Pelargonidin chloride

Delphinidin chloride

analytical standard

Peonidin 3-O-glucoside chloride

analytical standard

Cyanidin chloride

≥95% (HPLC)

Malvidin chloride

analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)

flow rate

0.6 mL/min

pressure

5440 psi (375 bar)

column temp.

35 ยฐC

detector

ESI(+), TOF

injection

1 μL

sample

20 μg/mL in 0.1% formic acid

์„ค๋ช…

๋ถ„์„ ๋ฉ”๋ชจ

Fruits of the genus Vaccinium are popular sources for anthocyanidin antioxidants. This genus includes the commonly known blueberry, huckleberry, and bilberry.

๋ฒ•์  ์ •๋ณด

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany