HPLC Analysis of Peptides on Discovery^® BIO Wide Pore C18 (Gradient #1)

column

Discovery BIO Wide Pore C18, 15cm × 4.6mm I.D., 5μm particles (568222-U)

mobile phase

[A] (80:20), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile

gradient

0-100% B in 14 min after 1 minute delay

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

10 μL

sample

Sigma Peptide Mix (Cat. No. P2693)