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HPLC Analysis of mPEG and mPEG-Peptide (Methoxy-PEG-Maleimide) on Zenixยฎ and Zenixยฎ-C SEC

HPLC Analysis of mPEG and mPEG-Peptide (Methoxy-PEG-Maleimide) on Zenix® and Zenix®-C SEC application for HPLC

Materials

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Z777033

Zenixยฎ SEC-300 Gel Filtration Column, 3 ฮผm

L ร— I.D. 30 cm ร— 7.8 mm, 3 μm particle size, stainless steel

Z777092

Zenixยฎ-C SEC-300 Gel Filtration Column, 3 ฮผm

L ร— I.D. 30 cm ร— 7.8 mm, 3 μm particle size, stainless steel

CONDITIONS

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)

column

Zenix-C SEC-300, 30 cm x 7.8 mm, I.D., 3 μm particles (Z777092)

mobile phase

150 mM sodium phosphate buffer, pH 7.0

flow rate

1.0 mL/min

column temp.

25 ยฐC

detector

UV, 214 nm

injection

20 μL

sample

20kD peptide mPEG-MAL (methoxy-PEG-Maleimide), 1 mg/mL

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Zenix is a registered trademark of Sepax Technologies

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