HPLC Analysis of Miconazole Enantiomers on Astec^® CYCLOBOND I 2000 HP-RSP

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] acetonitrile; [B] 20 mM ammonium acetate, pH 4.0; (25:75, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

miconazole, 1 mg/mL in acetonitrile:water (50:50)