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Merck

HPLC Analysis of Luciferin Enantiomers on CHIRALPAKยฎ AGP

HPLC Analysis of Luciferin Enantiomers on CHIRALPAK® AGP application for HPLC

Materials

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58150AST

CHIRALPAKยฎ AGP HPLC Column

5 μm particle size, L ร— I.D. 10 cm ร— 4 mm

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L6882

D-Luciferin sodium salt

lyophilized powder, synthetic

L6152

D-Luciferin

synthetic, BioXtra, ≥99% (HPLC)

L9504

D-Luciferin

synthetic

CONDITIONS

column

CHIRALPAK AGP, 10 cm x 4 mm I.D., 5 μm particles (58150AST)

mobile phase

10 mM sodium phosphate, pH 6.0

flow rate

0.9 mL/min

column temp.

25 ยฐC

detector

UV, 225 nm

sample

luciferin

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CHIRALPAK is a registered trademark of Daicel Corp.

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