HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis® Express 2.7 μm C18
Materials
분석 컬럼
제품 번호
설명
가격
표준
제품 번호
설명
가격
CONDITIONS
column
Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] water with 0.1% formic acid; [B] methanol
gradient
held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate
0.6 mL/min
pressure
4105 psi (283 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
20 μL
sample
20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)
설명
분석 메모
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany