HPLC Analysis of Elastase on Discoveryยฎ BIO PolyMA-SCX, Ion Exchange
Materials
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Elastase from porcine pancreas
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg proteinElastase from porcine pancreas
Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)CONDITIONS
column
Discovery BIO PolyMA-SCX, 5cm ร 4.6mm, 5μm particles (59601-U)
mobile phase
[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0
gradient
0 to 100% B in 30 min. (linear)
flow rate
0.5 mL/min
column temp.
25 ยฐC
detector
UV, 280 nm
injection
10 μL
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Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
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Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany