HPLC Analysis of Drugs of Abuse on Ascentis^® Si (HILIC Mode)

column

Ascentis Si, 5 cm x 4.6 mm I.D., 5 μm particles (581511-U)

mobile phase

[A] 0.1% ammonium acetate in water (pH unadjusted): [B] 0.1% ammonium acetate in acetontrile; (10:90, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

10 μL

sample

as indicated in 0.1% ammonium acetate in 10:90:water:acetonitrile