HPLC Analysis of Dehydroascorbic Acid and Ascorbic Acid (Vitamin C) on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 μm, 200 Å (1.50455.0001)

mobile phase

[A] acetonitrile; [B] ammonium acetate; (70:30, A,B)

flow rate

0.5 mL/min

pressure

375 psi (2.6 MPa)

detector

240 nm

injection

5 μL