HPLC Analysis of Cyclophosphamide Enantiomers on Astec® CHIROBIOTIC® T
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] ethanol; [C] THF; (90:5:5, A:B:C)
flow rate
0.75 mL/min
column temp.
23 °C
detector
UV, 205 nm
injection
5 μL
sample
1 mg/mL in methanol
설명
법적 정보
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC