HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)
CONDITIONS
column
Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)
mobile phase
[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)
flow rate
0.6 mL/min
column temp.
25 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
2 μL
sample
1000 μg/mL in 10:90, water: acetonitrile
설명
분석 메모
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany