HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] 0.1% sodium phosphate, pH 4.1; [B] methanol; (60:40, A:B)
flow rate
1 mL/min
column temp.
23 °C
detector
UV, 210 nm
injection
2 μL
sample
4 mg/mL in methanol
설명
법적 정보
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC