GC Analysis of Tricyclic Antidepressants in Urine on a 100% Methyl Column after SPME using 100 μm PDMS Fiber

sample/matrix

1 mL urine (1 μg each analyte + 50 μL 5 M NaOH) in 7.5 mL vial

SPME fiber

polydimethylsiloxane, 100 μm (57300-U)

extraction

headspace, 15 min, 100 °C (sample incubated 30 min)

desorption process

3 min, 280 °C

column

100% methyl polysiloxane, 30 m × 0.32 mm I.D., 0.25 μm (Supelco equivalent, Equity-1, 28055-U)

oven

100 °C to 300 °C at 20 °C/min

inj. temp.

splitless (splitter opened after 1 min), 280 °C

detector

FID, 280 °C

carrier gas

helium, 30 cm/sec