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Merck

LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astecยฎ CHIROBIOTICยฎ R

LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R application for HPLC

Materials

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13019AST

Astecยฎ CHIROBIOTICยฎ R Chiral HPLC Column

5 μm particle size, L ร— I.D. 15 cm ร— 2.1 mm

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73594

Ammonium acetate

LiChropurโ„ข, eluent additive for LC-MS

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L0625

D-(โˆ’)-Lactic acid

≥90%

46937

L-(+)-Lactic acid

analytical standard

CONDITIONS

column

Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 μm particles (13019AST)

mobile phase

[A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)

flow rate

0.7 mL/min

column temp.

4 ยฐC

detector

(-)ESI

injection

20 μL

sample

Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H2O and 85% (v/v) acetonitrile

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Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.

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Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatographyโ€“tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Bรฉguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.

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Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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