GC Analysis of cis/trans FAME Column Performance Mix on SP^®-2560 per AOAC Method Ce 1h-05

Materials

분석 컬럼

제품 번호

설명

가격

SP^®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, d_f 0.20 μm

표준 물질

제품 번호

설명

가격

cis/trans F.A.M.E Column Performance Mix

2.5 mg/mL in dichloromethane, ampule of 1 mL

CONDITIONS

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

180 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 1 mL/min

injection

1 μL, 100:1 split

sample

cis/trans FAME Column Performance Mix (40495-U)

설명

분석 메모

Complex mixtures of cis/trans FAME isomers are difficult to separate. Baseline resolution may not be possible for some samples due to variation in GC instrument designs and column efficiencies. As a result, AOAC Method Ce 1h-05 recommends monitoring changes in column performance with a known FAME mixture closely matching the range of fatty acids under investigation.

The resolution factor for Peak 1 and Peak 2 of this mix (40495-U) is a good indicator of colum performance. Using one of the resolution equations listed on the Data Sheet, the resolution factor can be calculated. Resolution factors approaching 1.5 (baseline resolution) are desired.

For additional information, request Data Sheet T708019.

법적 정보

SP is a registered trademark of Sigma-Aldrich Co. LLC

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GC Analysis of cis/trans FAME Column Performance Mix on SP®-2560 per AOAC Method Ce 1h-05