1X Phosphate-Buffered Saline (PBS) Recipe Calculator
Preparing PBS 1X by Volume
Phosphate-buffered saline (PBS) is an isotonic solution that is used in many biological research applications. To make 1 L of PBS, add 100 mL of 10X PBS to 900 mL of water. This PBS recipe contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4.
This calculator enables the accurate preparation of this 1X PBS wash buffer for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed to make this common buffer used for Western blotting and other immunoassays.
1X PBS Solution Concentrations:
- NaCl: 137 mM
- KCl: 2.7 mM
- Na2HPO4: 10 mM
- KH2PO4: 1.8 mM
PBS Recipe Calculator
| 1 L Recipe | Desired Volume: mL |
| 100 mL of 10X PBS Stock Solution | mL of 10X PBS Stock Solution |
| 900 mL of Milli-Q® Water | mL of Milli-Q® Water |
| The resultant 1X PBS has a final concentration of 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl; pH 7.4. |
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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