G1006856

UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell A160 Peptide C18 Using Difluoroacetic Acid as a Mobile Phase Modifier

application for UHPLC

속성

• 기술: UHPLC: suitable
• 검사 파라미터: column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U); mobile phase: [A] 75:25 water (0.1% v/v difluoroacetic acid): acetonitrile (0.1% v/v difluoroacetic acid); [B] 50:50 water (0.1% v/v difluoroacetic acid): acetonitrile (0.1% v/v difluoroacetic acid); (77:23, A:B); flow rate: 0.2 mL/min; column temp.: 75 °C; injection: MS, ESI(+) TOF, TIC 100-3000 m/z; injection: 0.5 μL; sample: Mixture of six insulin variants, 100 μg/mL, water (0.1% v/v difluoroacetic acid)
• 적합성: application for UHPLC
• 응용 분야: life science and biopharma

설명

분석 메모

BIOshell A160 Peptide C18 is an ideal column for reversed-phase separations of small proteins to large peptide sequences. There is over 75% sequence similarity between all six of the insulin variants in the sample; therefore, separation of these variants is achieved through the subtle differences in the polarities of surface residues on the peptides. The use of difluoroacetic acid (DFA) has less of an effect on ion suppression than trifluoroacetic acid (TFA), thus allowing for mass spectral detection of the insulin variants.

법적 정보

분석물

insulin-Glargine
insulin-Bovine
insulin-Asp
insulin-Lispro
insulin-Human
insulin-Porcine