기술
HPLC: suitable
검사 파라미터
column: Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase: [A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 1350 ppi (93 bar)
column temp.: 25 °C
detector: MS, ESI (+), TIC, m/z 150-300
injection: 5 μL
적합성
application for HPLC
응용 분야
chemicals and industrial polymers
분석 메모
L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
분석물
설명
L-Methionine sulfone
L-Methionine sulfoximine, suitable for cell culture
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시험 성적서(COA)
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