G000595

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery^® C18 after SPE using Discovery^® DSC-18

application for SPE, application for HPLC

속성

• 기술: HPLC: suitable; solid phase extraction (SPE): suitable
• 검사 파라미터: sample preparation: SPE (Solid Phase Extraction); SPE tube/cartridge: Discovery DSC-18, 100 mg/1 mL (52602-U); column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm C18 guard column and 0.5 μm frit filter (504955); mobile phase: [A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C); flow rate: 1 mL/min; column temp.: ambient; detector: UV, 254 nm; injection: 50 μL, diluted porcine serum extract
• 적합성: application for HPLC; application for SPE
• 응용 분야: clinical

설명

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Nortriptyline
Doxepin
Imipramine
Amitriptyline hydrochloride
Trimipramine solution