추천 제품
product name
TSKgel® Octadecyl-4PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 7 μm particle size
material
stainless steel
제품 라인
TSKgel®
제조업체/상표
Tosoh 13351
라벨링 범위
1 meq/mL ligand density (estimate)
파라미터
no limitation salt concentration
0-100% organic solvent
1.2 mL/min flow rate
120 bar max. pressure
5-50 °C temperature
기술
HPLC: suitable
길이 × I.D.
15 cm × 4.6 mm
기질
polymer particle platform
기질 활성군
C18 (octadecyl) phase
입자 크기
7 μm
공극 크기
500 Å pore diameter
작동 pH
2-12
분리 기술
reversed phase
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일반 설명
Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500Ã… pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
- Octadecyl-2PW particles have 125Å pores
- Octadecyl-4PW particles have 500Å pores
- Phenyl-5PW RP particles have 1000Å pores
- Octadecyl-NPR particles are nonporous
물리적 형태
Shipped in 60% methanol - 40% water.
법적 정보
TSKgel is a registered trademark of Tosoh Corporation
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