추천 제품
product name
SUPELCOSIL™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Agency
suitable for USP L3
제조업체/상표
SUPELCOSIL™
파라미터
≤70 °C temp. limit
400 bar pressure (5801 psi)
기술
HPLC: suitable
길이 × I.D.
5 cm × 4.6 mm
표면적
170 m2/g
기질
silica gel, spherical particle platform
기질 활성군
silica phase
입자 크기
5 μm
공극 크기
120 Å pore size
pH 범위
2-7.5
응용 분야
food and beverages
호환성
mode of use normal phase HPLC
분리 기술
hydrophilic interaction (HILIC)
normal phase
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일반 설명
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.
법적 정보
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Choose from one of the most recent versions:
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however
J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm
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