ZRB2789

Anti-CD11a Antibody, clone 2J15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): Anti-FLT4 Antibody, clone 2F4 ZooMAb^® Rabbit Monoclonal, Integrin alpha-L (UniProt: P20701, also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 2J15, recombinant monoclonal
• 설명: recombinant, expressed in HEK 293 cells
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 128.77 kDa; observed mol wt ~180 kDa
• 정제법: using Protein A
• 종 반응성: human
• 포장: antibody small pack of 25
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG
• 에피토프 서열: C-terminal half
• 단백질 ID 수납 번호: NP_002200
• UniProt 수납 번호: P20701
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 유전자 정보: human ... ITGAL(3683)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 2J15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD11a. It targets an epitope within 23 amino acids from the C-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human CD11a.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD11a in THP-1 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD11a in lysates from Jurkat cells and Human spleen tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD11a in Human spleen tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD11a in one million Human peripheral blood mononuclear cells (PBMC)..

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD11a in MV4-11 cells.

Affinity Binding Assay: A representative lot of this antibody bound in CD11a peptide with a KD of 2.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Integrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are heterodimeric, integral membrane proteins composed of a variable -subunit and a conserved β-subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the -subunit. CD11a is a single-pass type I membrane glycoprotein that noncovalently associates with β2 integrin to forms a heterodimer and serve as a receptor for IC

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable