콘텐츠로 건너뛰기
Merck
모든 사진(3)

주요 문서

ZRB2510

Sigma-Aldrich

Anti-GPx4 Antibody, clone 1G5 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

동의어(들):

EC:1.11.1.12, EC:1.11.1.9, GPx-4, GSHPx-4, Glutathione Peroxidase 4, PHGPx, Phospholipid hydroperoxide glutathione peroxidase GPX4

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203

생물학적 소스

rabbit

Quality Level

재조합

expressed in HEK 293 cells

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

1G5, recombinant monoclonal

설명

recombinant, expressed in HEK 293 cells

제품 라인

ZooMAb® learn more

형태

lyophilized

분자량

calculated mol wt 22.18 kDa
observed mol wt ~18 kDa

정제법

using Protein A

종 반응성

human, mouse, rat

종 반응성(상동성에 의해 예측)

monkey, chimpanzee

포장

antibody small pack of 25

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

기술

affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG

에피토프 서열

C-terminal

단백질 ID 수납 번호

UniProt 수납 번호

배송 상태

ambient

저장 온도

2-8°C

일반 설명

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 1G5 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects GPx4. It targets an epitope within 22 amino acids from the C-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human GPX4.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in U-937 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GPx4 in U-937 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected GPx4 in lysate from mouse liver and rat testis tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GPx4 in human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound GPx4 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Phospholipid hydroperoxide glutathione peroxidase, cytosolic (UniProt: P36969; also known as PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4) is encoded by the GPX4 gene (Gene ID: 2879) in human. The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. This selenocysteine is crucial for the enzyme′s catalytic activity. Sec is encoded by the UGA codon, which normally signals translation termination. Three functionally distinct isozymes of GPx4 have been described and are known as mGPx4 (mitochondrial), cGPx4 (cytosolic), and nGPx4 (nuclear). They originate from a single GPX4 gene. The cytosolic isoform is ubiquitously expressed at moderate levels in most mammalian cells. On the other hand, mitochondrial and nuclear isoforms are overexpressed in spermatoid cells. GPx4 is a considered to be a ′moonlighting′ protein because of its ability to serve dual functions as a peroxidase, that has been implicated in anti-oxidative defense, gene expression regulation, and programmed cell death, and as a structural protein in spermatogenesis. Mutations or deficiencies in GPX4 are associated with several diseases. One of the most severe conditions linked to GPX4 deficiency is Sedaghatian-type spondylometaphyseal dysplasia (SMDS), a rare genetic disorder characterized by skeletal abnormalities, neurological impairment, and early infant death. Homozygous GPx4-deficient mice are reported to die utero before E7.5. Similarly, homozygous mice expressing catalytically inactive GPx4 variants (Sec46Ala/Ser mutant) do not survive beyond the 7th day of embryogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Borchert, A., et al (2010). Immunol. Lett. 133 (2); 85-93).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.