추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2H10, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt ~97.21 kDa
observed mol wt ~97 kDa
종 반응성
rat, human, mouse
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 2H10 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein Argonaute -2 (Ago2). It targets an epitope within 23 amino acids from the N-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human Protein argonaute-2 (Ago2).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in MCF-7, NIH3T3, and C6 cell lysates.
Flow Cytometry Analysis: 1 μg from a representative lot detected Ago2 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ago2 in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in MCF-7, NIH3T3, and C6 cell lysates.
Flow Cytometry Analysis: 1 μg from a representative lot detected Ago2 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ago2 in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ago2 in NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Ago2, clone 2H10 ZooMAb®, Cat. No. ZRB1205, is a rabbit recombinant monoclonal that specifically targets Ago2 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
표적 설명
Protein argonaute-2 (UniProt: Q9UKV8; also known as Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C, eIF-2C, eIF2C, PAZ Piwi domain protein, PPD, Protein slicer) is encoded by the AGO2 (also known as EIF2C2) gene (Gene ID: 27161) in human. Ago2 is a highly conserved member of the AGO family of proteins with catalytic activity and is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by Ago2 and binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation and is independent of endonuclease activity. Ago2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. Ago2 is shown to have four core domains that are known as N domain, PAZ domain (aa 235-348), MID domain, and PIWI domain (aa 517-818). The PAZ domain is an RNA binding module that recognizes the 3 end of both siRNA and miRNA, in a sequence independent manner and is reported to be strictly required for Ago2 to form the RISC complex. The PIWI domain has major roles within Ago2, including RNase H like endonucleolytic activity and regulating the interaction between MID domain and DNA/RNA. Ago2 is shown to undergo 4-hydoxylation that enhances its stability but is not essential for miRNA binding or endonuclease activity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ye, Z., et al. (2015). J. Cancer 6(9); 877-882).
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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