생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
2B15, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt ~39.93 kDa
observed mol wt ~40 kDa
종 반응성
rat, human, mouse
종 반응성(상동성에 의해 예측)
bovine, monkey
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 2B15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 3 subunit H (elF3h). It targets an epitope within 21 amino acids from the C-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Eukaryotic translation initiation factor 3 subunit H (elF3h).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF3h in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF3h in A431, PC12, and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF3h in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected eIF3h in A431 and HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound eIF3H peptide with a KD of 1.1 x 10-6 in an Affinity Binding Assay,
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF3h in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF3h in A431, PC12, and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF3h in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected eIF3h in A431 and HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound eIF3H peptide with a KD of 1.1 x 10-6 in an Affinity Binding Assay,
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This rabbit recombinant monoclonal Anti-eIF3h, clone 2B15 ZooMAb® Rabbit Monoclonal, Cat. No. ZRB1111 is used in Western Blotting, Affinity Binding Assay, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of eIF3h.
표적 설명
Eukaryotic translation initiation factor 3 subunit H (UniProt: O15372; also known as eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit) is encoded by the EIF3H (also known as EIF3S3) gene (Gene ID: 8667) in human. elF3h is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex that is required for several steps in the initiation of protein synthesis. The elF3 complex is composed of 13 different subunits that are divided into three modules. elF3h belongs to module B that also contains elF3f and elf3M. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex is reported to specifically target and initiate translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.