추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1D19, recombinant monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt 13.83 kDa
observed mol wt ~14 kDa
종 반응성
human
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
동형
IgG
에피토프 서열
Unknown
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
특이성
Clone 1D19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Phosphohistidine phosphatase 1 (PHTP1).
면역원
His-tagged full-length recombinant human Phosphohistidine phosphatase 1 (PHPT1).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHPT1 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHPT1 in A549 cell lysate.
ELISA Analysis: 300 μg/mL from a representative lot detected PHPT1 protein in an indirect ELISA.
Affinity Binding Assay:: A representative lot of this antibody bound PHPT1 protein with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHPT1 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHPT1 in A549 cell lysate.
ELISA Analysis: 300 μg/mL from a representative lot detected PHPT1 protein in an indirect ELISA.
Affinity Binding Assay:: A representative lot of this antibody bound PHPT1 protein with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PHPT1, clone 1D19 ZooMAb®, Cat. No. ZRB1056, is recombinant Rabbit monoclonal antibody that detects phosphohistidine phosphatase 1 (PHPT1) and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting.
표적 설명
14 kDa phosphohistidine phosphatase (UniProt: Q9NRX4; also known as EC:3.9.1.3, Phosphohistidine phosphatase 1, PHPT1, Protein histidine phosphatase, PHP, Protein janus-A homolog) is encoded by the PHPT1 (also known as PHP14, CGI-202, HSPC141) gene (Gene ID: 29085) in human. Protein histidine phosphorylation is reported to exist widely in vertebrates and PHPT is the only phosphohistidine phosphatase described in mammals. It regulates phosphohistidine levels of several proteins including those involved in signaling, lipid metabolism, and potassium ion transport. It is expressed abundantly in heart and skeletal muscle and is also found in several other tissues. Human PHPT1 is composed of six β-strands that are flanked by two -helices. The substrate-binding site is shown to be positioned between two loops, one between 1 and β4, and the other between β5 and 2. This site contains multiple residues that are shown to be important for substrate binding and include Glu51, Tyr52, His53, Tyr93, and Met95. The active site His53, located at the beginning of helix 1, is identified as the catalytic residue and the NH groups of His53, Ala54, and Ala86 and the OH group of Ser94 act as anchors of substrate and stabilize phosphate binding to the active site. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Martin, DR., et al. (2016). Sci. Rep. 6; Article 23658; Gong, W. B. et al. Biochem. J. 418(2); 337-344; Busam, R. D. et al. (2006). J. Biol. Chem. 281(45); 33830-33834).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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