추천 제품
사용
sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
특징
dNTPs included
hotstart
기술
PCR: suitable
배송 상태
dry ice
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
The REDExtract-N-Amp™ Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by direct PCR (polymerase chain reaction). A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
애플리케이션
REDExtract-N-Amp™ seed PCR Kit has been used for DNA extraction and to amplify hygromycin resistance gene.(hpt) by polymerase chain reaction (PCR).
특징 및 장점
- Perfect for quick genotyping results
- Rapid extraction of genomic DNA for PCR in 15 minutes
- No long enzymatic digestions
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- Compatible with a wide variety of seed sources
- Extracts are stable at 4 °C for at least 6 months, allowing for multiple reassays
- Phenol/chloroform extraction or alcohol precipitation not required
- Column purification or centrifugation not required
원리
The kit comes with validated protocols to extract and amplify genomic DNA from corn, wheat, sunflower, cotton, soybean, sorghum, canola, and Arabidopsis seeds. Additional protocols are available. In a typical procedure, genomic DNA is extracted from a sample that has been ground and incubated in the seed preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR Reaction Mix (included in the kit) and PCR is initiated.
기타 정보
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
법적 정보
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR is a registered trademark of Molecular Probes, Inc.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 전용
제품 번호
설명
- Seed Preparation Solution
관련 제품
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Sadiye Hayta et al.
Current protocols, 1(3), e58-e58 (2021-03-04)
Wheat, though a key crop plant with considerable influence on world food security, has nonetheless trailed behind other major cereals in the advancement of gene transformation technology for its improvement. New breeding technologies such as genome editing allow precise DNA
Marko Stojkovic et al.
Journal of separation science, 35(24), 3509-3513 (2012-12-12)
The use of CE with contactless conductivity detection for the determination of PCR products is demonstrated for the first time. The separation of specific length PCR products according to their size could be achieved using 5% PVP as a sieving
Angela Quarta et al.
FEMS microbiology letters, 259(1), 7-13 (2006-05-11)
The ability to rapidly distinguish trichothecene chemotypes in a given species/population of the genus Fusarium is important due to significant differences in the toxicity of these secondary metabolites. A multiplex PCR assay, based on primer pairs derived from the Tri3
문서
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
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